![]() When an antibiotic has been added to the extender, the semen extender mixture should not be used for at least 20 minutes to allow sufficient time for the antibiotics to control the penile pathogens which may be sensitive to the antibiotics. Add Canine Semen Extender (at 37.8✬ (100☏)) to the semen and maintain at a temperature of 37.8✬ (100☏) for no more than two hours before use. ![]() Another approach is to dilute the semen so that each insemination dose contains 200 million motile spermatozoa.A ratio of 1 part semen to 4 parts extender is generally recommended, but ratios between 1:1 and 1:4 are acceptable.Synbiotics (Zoetis), CaniPlus Chill 10, CaniPlus Chill LT and CaniPlus Chill ST (Minitube. Decide on a desired ratio of semen to extender. for canine semen extenders (PONGLOWHAPAN et al., 2004).Before mixing semen with extender, the extender must be at 37.8✬ (100☏) to avoid cold shock to the spermatozoa.Higher thawing temperatures my cause ingredient damage. Thaw frozen extender by placing the bottle in a water bath or an incubator at 37.8✬ (100☏).The mixed extender may be stored in an incubator or water bath at 37.8✬ (100☏) or it can be frozen (in smaller portions, if desired) in sterile containers at a temperature of <20✬ (-5☏) for up to 6 months. For best results, raise the temperature of the sterile water to 37.8✬ (100☏) by placing the bottle in an incubator or a water bath prior to adding the powder.Add the processed powder to the sterile water.Mixing Instructions of the Canine Semen Extender: MINITUBE 25.5' Flexible Insemination Pipette (100/pkg) Price: 240.00. Do not hold components from one breeding season to the next. Canine Extenders Canine Freezing Supplies Densimeters (Sperm Counters) Pipette and Tips. Do not expose to elevated temperatures, high humidity or sunlight. ![]()
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